Primer

Part:BBa_K5427018

Designed by: Brittany Green   Group: iGEM24_UAlberta   (2024-09-23)


FW Keratin Pet22B+

Forward primer for amplification of keratin31 insert in pET22b+.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 38
    Illegal XbaI site found at 55
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 38
    Illegal NotI site found at 45
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 38
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 38
    Illegal XbaI site found at 55
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 38
    Illegal XbaI site found at 55
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None